TA503052

antibody from OriGene

Targeting: ITFG2 FGGAP1, MDS028

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503052

Provider

OriGene

Proper citation

OriGene Cat#TA503052, RRID:AB_11127986

Product name

ITFG2 (MDS028) mouse monoclonal antibody, clone OTI 3B3 (formerly 3B3)

Antibody type

Monoclonal

Description

ITFG2 (MDS028) mouse monoclonal antibody, clone OTI 3B3 (formerly 3B3)

Host

Mouse

Conjugate

Unconjugated

Epitope

ITFG2

Isotype

IgG

Antibody clone number

OTI 3B3

Vial size

100 µl

Concentration

0.33 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ITFG2 (RC200818, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ITFG2.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ITFG2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-ITFG2 mouse monoclonal antibody (TA503052) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ITFG2(RC200818).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC200818 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ITFG2 antibody(TA503052), and then analyzed by flow cytometry.

Validation comment

FC