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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-80443 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD79b Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human CD79B/Ig-beta.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CD79b Polyclonal Antibody (Product # PA5-80443) at 1:500 dilution. Lane A: Daudi Whole Cell Lysate, Lane B: Hela Whole Cell Lysate, Lane C: Mouse liver Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 33 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of cd79b in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with CD79b Polyclonal Antibody (Product # PA5-80443, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane and cytoplasm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of cd79b in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with CD79b Polyclonal Antibody (Product # PA5-80443, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane and cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CD79b Immunoprecipitation using: Lane A: 0.5 mg Daudi Whole Cell Lysate 4 µL with CD79b Polyclonal Antibody (Product # PA5-80443) and 15 µL of 50 % Protein G agarose. Primary antibody: CD79b Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 33 kDa.
