PA5-80443

antibody from Invitrogen Antibodies

Targeting: CD79B B29, IGB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80443

Provider

Invitrogen Antibodies

Product name

CD79b Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CD79b Polyclonal Antibody (Product # PA5-80443) at 1:500 dilution. Lane A: Daudi Whole Cell Lysate, Lane B: Hela Whole Cell Lysate, Lane C: Mouse liver Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 33 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of cd79b in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with CD79b Polyclonal Antibody (Product # PA5-80443, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane and cytoplasm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of cd79b in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with CD79b Polyclonal Antibody (Product # PA5-80443, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane and cytoplasm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD79b Immunoprecipitation using: Lane A: 0.5 mg Daudi Whole Cell Lysate 4 µL with CD79b Polyclonal Antibody (Product # PA5-80443) and 15 µL of 50 % Protein G agarose. Primary antibody: CD79b Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 33 kDa.