PA5-18290

antibody from Invitrogen Antibodies

Targeting: DLC1 ARHGAP7, DLC-1, HP, p122-RhoGAP, STARD12

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-18290

Provider

Invitrogen Antibodies

Product name

DLC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse and porcine based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.

Reactivity

Human, Mouse

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

Functional antagonism between CagA and DLC1 in gastric cancer.

Hinsenkamp I, Köhler JP, Flächsenhaar C, Hitkova I, Meessen SE, Gaiser T, Wieland T, Weiss C, Röcken C, Mowat M, Quante M, Taxauer K, Mejias-Luque R, Gerhard M, Vogelmann R, Meindl-Beinker N, Ebert M, Burgermeister E
Cell death discovery 2022 Aug 13;8(1):358

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of DLC1 using DLC1 Polyclonal Antibody (Product # PA5-18290) in paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of DLC1 using DLC1 Polyclonal Antibody (Product # PA5-18290) in paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Negative Control for DLC1 Polyclonal Antibody (Product # PA5-18290), showing immunohistochemical staining of paraffin embedded Mouse Adrenal Gland, with no primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (PFA fixed) analysis of DLC1 using DLC1 Polyclonal Antibody (Product # PA5-18290) (4 µg/mL) in staining of paraffin embedded Mouse Adrenal Gland. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of DLC1 mRNA/protein variants in human GC cells. A Scheme of human DLC1 mRNA/protein variants. FL DLC1v1 and the N-terminally truncated DLC1v4 isoform exhibit identical C-terminal multi-domain organisation: sterile-alpha motif (SAM), serine-rich region, nuclear localisation sequence (NLS), caveolin-1 (CAV1)-binding motif (Cavbm), RHO GTPase-activating protein (GAP), and steroidogenic acute regulatory protein-related lipid-transfer (START) domains. DLC1v4 (start codon: MKLEI, aa 513-1528) used in the present study lacks the SAM region compared to DLC1v1 (aa 1-1528). B Comparison of putative driver splice mutations in the human FL DLC1v1 gene located N- and C-terminal of the SAM domain where the DLC1v4 transcript starts. Point mutation data were retrieved from cBioPortal (Table S 6 ) based on COSMIC/GISTIC entries. Cancer hotspots (red) and splice variants (yellow) are similar in human cancer cell lines (CCLE) and pan-cancer studies (from patients with EC/GC). C Detection of DLC1 mRNA variants. Total RNA from human gastrointestinal cancer and non-cancer (HEK293T) cell lines were subjected to RT-qPCR using primers directed against N- and C-terminal regions of the FL DLC1v1 cDNA. Quantitative analyses (top) and representative images from PCR-amplification products upon ethidium bromide-stained agarose gel electrophoresis (bottom). Ct-values were normalised to B2M and calculated as -fold +- S.E. (* p < 0.05 N vs. C, 2way-ANOVA with Bonferroni post-tests, n = 3 per cell