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LearnPA5-28443
antibody from Invitrogen Antibodies
Targeting: DLC1
ARHGAP7, DLC-1, HP, p122-RhoGAP, STARD12
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-28443 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DLC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: H1299, HeLaS3, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (100%), Dog (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.84 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Aberrant DNA methylation of alternative promoter of DLC1 isoform 1 in meningiomas.
Bujko M, Kober P, Rusetska N, WakuĊa M, Goryca K, Grecka E, Matyja E, Neska J, Mandat T, Bonicki W, Siedlecki JA
Journal of neuro-oncology 2016 Dec;130(3):473-484
Journal of neuro-oncology 2016 Dec;130(3):473-484
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DLC1 Polyclonal Antibody (Product # PA5-28443). Sample (30 µg of whole cell lysate). A: MOLT4SDS PAGE. Z. 7.5% SDS PAGE. DLC1 Polyclonal Antibody (Product # PA5-28443) diluted at 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Pheochromocytoma xenograft, using DLC1 (Product # PA5-28443) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 a Examples of immunohistochemical staining of DLC1-v1/-v3 protein expression in meningiomas. Magnification x400. b Comparison of DNA methylation levels in meningiomas with high/moderate DLC1 expression and meningiomas with none/poor DLC1 expression. c Comparing the relative expression levels of DLC1v1 and DLC1v3 measured by 220511_s_at probeset (HG U133A Array, Affymetrix), GSE43290 dataset. Horizontal lines represent the mean value
