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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MA5-23991 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MEP1B Monoclonal Antibody (289731)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody detects the pro form of human or mouse Meprin beta Subunit/MEP1B in Western blots. This antibody does not react with the mature form. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 289731
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human intestine tissue and mouse intestine tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-human/mouse Meprin ß Subunit/MEP1B Monoclonal Antibody (Product # MA5-23991) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for Meprin ß Subunit/MEP1B at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MEP1B in human intestine tissue and mouse intestine tissue. Samples were incubated in MEP1B monoclonal antibody (Product # MA5-23991) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Rat IgG secondary antibody. A specific band was detected for Meprin β Subunit/MEP1B at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MEP1B in immersion fixed paraffin-embedded sections of human intestine. Samples were incubated in MEP1B monoclonal antibody (Product # MA5-23991) using a dilution of 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hemotoxylin (blue). Specific staining was localized to the Brush border of epithelial cells This application has not been tested in mouse samples.
