PA5-21977

antibody from Invitrogen Antibodies

Targeting: TEAD4 EFTR-2, RTEF-1, TCF13L1, TEF-3, TEFR-1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21977

Provider

Invitrogen Antibodies

Product name

TEAD4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2, HepG2 nuclear extract. Predicted reactivity: Mouse (91%), Pig (94%), Chicken (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TEAD4 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TEAD4 Polyclonal Antibody (Product # PA5-21977) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TEAD4 Polyclonal Antibody detects TEAD4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TEAD4 stained by TEAD4 Polyclonal Antibody (Product # PA5-21977) diluted at 1:1,000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using TEAD4 (Product # PA5-21977) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.