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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- TA326504 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal Anti-Synaptotagmin-7 Antibody
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal Anti-Synaptotagmin-7 Antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- Syt7
- Isotype
- IgG
- Antibody clone number
- S275-14
- Vial size
- 100 µg
- Concentration
- 1mg/mL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- WB of rat brain lysate showing detection of ~65 kDa Synaptotagmin 7 protein using Anti-Synaptotagmin 7. Lane 1: MW ladder. Lane 2: Anti-Synaptotagmin 7 (1:100). Lane 3: Anti-Synaptotagmin 7 (1:250). Lane 4: Anti-Synaptotagmin 7 (1:500). Lane 5: Anti-Synaptotagmin 7 (1:1000). Load: 20 ug RB lysate per lane. Block: 5% milk + TBST 1hr at RT. Primary antibody: Mouse Anti-Synaptotagmin 7 monoclonal antibody incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody at 1:50 for 60 min at RT. Colour Development: TMB solution for 30 min at RT. Predicted/Observed size: ~ 65 kDa. Other band(s) (faint): ~25kDa, ~37kDa, ~100kDa, ~150kDa.
- Validation comment
- WB
