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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MA5-27654 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Synaptotagmin 7 Monoclonal Antibody (N275/14)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- 1 µg/mL of MA5-27654 was sufficient for detection of Synaptotagmin-7 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 45kDa. Does not cross-react with Synaptotagmin-6 (or others). Can identify other isoforms bands at approximately 65kD. This antibody was formerly sold as clone S275-14.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- N275/14
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references IL-2 Inducible Kinase ITK is Critical for HIV-1 Infection of Jurkat T-cells.
Hain A, Krämer M, Linka RM, Nakhaei-Rad S, Ahmadian MR, Häussinger D, Borkhardt A, Münk C
Scientific reports 2018 Feb 16;8(1):3217
Scientific reports 2018 Feb 16;8(1):3217
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Synaptotagmin 7 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with Synaptotagmin 7 monoclonal antibody (Product # MA5-27654) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Synaptotagmin-7 Antibody, D) Merged image. Magnification: 60x.
