MA5-23246

antibody from Invitrogen Antibodies

Targeting: RNF212 FLJ38841, LOC285498

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23246

Provider

Invitrogen Antibodies

Product name

RNF212 Monoclonal Antibody (5H3)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Peptide Sequence: IKSSVSTKPH GCLLPPHSSA PDRLESMEVD LSPSPIRKSE IAAGPARISM ISPPQDGRMA PCARRVCHFQ RFTMFLHRRL SSLAAPPSVQ FWKARGTHQL

Antibody clone number

5H3

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RNF212 expressed in transfected 293T cell line by anti-RNF212 monoclonal antibody (Product # MA5-23246), clone 5H3. Lane 1: RNF212 transfected lysate (Predicted MW: 33.4 KDa). Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RNF212 expressed in transfected 293T cell line by anti-RNF212 monoclonal antibody (Product # MA5-23246), clone 5H3. Lane 1: RNF212 transfected lysate (Predicted MW: 33.4 KDa). Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RNF212 using anti-RNF212 monoclonal antibody (Product # MA5-23246) is 0.1 ng/mL as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using an anti-RNF212 monoclonal antibody (Product # MA5-23246). (antibody concentration 10 µg/mL).