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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MA5-23246 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF212 Monoclonal Antibody (5H3)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Peptide Sequence: IKSSVSTKPH GCLLPPHSSA PDRLESMEVD LSPSPIRKSE IAAGPARISM ISPPQDGRMA PCARRVCHFQ RFTMFLHRRL SSLAAPPSVQ FWKARGTHQL
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5H3
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RNF212 expressed in transfected 293T cell line by anti-RNF212 monoclonal antibody (Product # MA5-23246), clone 5H3. Lane 1: RNF212 transfected lysate (Predicted MW: 33.4 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RNF212 expressed in transfected 293T cell line by anti-RNF212 monoclonal antibody (Product # MA5-23246), clone 5H3. Lane 1: RNF212 transfected lysate (Predicted MW: 33.4 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged RNF212 using anti-RNF212 monoclonal antibody (Product # MA5-23246) is 0.1 ng/mL as a capture antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using an anti-RNF212 monoclonal antibody (Product # MA5-23246). (antibody concentration 10 µg/mL).
