Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-83250 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83250, RRID:AB_11009242
- Product name
- Rabbit Polyclonal HFE Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human HFE antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Hemochromatosis enhances tumor progression via upregulation of intracellular iron in head and neck cancer.
Lenarduzzi M, Hui AB, Yue S, Ito E, Shi W, Williams J, Bruce J, Sakemura-Nakatsugawa N, Xu W, Schimmer A, Liu FF
PloS one 2013;8(8):e74075
PloS one 2013;8(8):e74075
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HFE Antibody [NBP1-83250] - Analysis in control (vector only transfected HEK293T lysate) and HFE over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HFE Antibody [NBP1-83250] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: HFE Antibody [NBP1-83250] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HFE Antibody [NBP1-83250] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HFE Antibody [NBP1-83250] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.