PA5-59087

antibody from Invitrogen Antibodies

Targeting: SPON2 DIL1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-59087

Provider

Invitrogen Antibodies

Product name

SPON2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Immunogen sequence: HPANSFYYPR LKALPPIARV TLLRLRQSPR AFIPPAPVLP SRDNEIVDSA SVPETPLDCE VSLWSSWGLC GGHCGRLGTK SRTRYVRVQP ANNGS Highest antigen sequence identity to the following orthologs: Mouse - 83%, Rat - 84%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Expression of Extracellular Matrix-Related Genes and Their Regulatory microRNAs in Problematic Colorectal Polyps.

Žlajpah M, Boštjančič E, Tepeš B, Zidar N
Cancers 2020 Dec 11;12(12)

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Hippo Signaling Plays an Essential Role in Cell State Transitions during Cardiac Fibroblast Development.

Xiao Y, Hill MC, Zhang M, Martin TJ, Morikawa Y, Wang S, Moise AR, Wythe JD, Martin JF
Developmental cell 2018 Apr 23;45(2):153-169.e6

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of SPON2 in human placenta using SPON2 Polyclonal Antibody (Product # PA5-59087) shows moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of SPON2 in human prostate cancer using SPON2 Polyclonal Antibody (Product # PA5-59087) shows strong cytoplasmic positivity in tumor cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of SPON2 in human lymph node using SPON2 Polyclonal Antibody (Product # PA5-59087) shows no positivity in non-germinal center cells as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of SPON2 in human pancreas using SPON2 Polyclonal Antibody (Product # PA5-59087) shows low positivity in exocrine glandular cells as expected.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 ( a ) Adenoma; ( b ) adenoma with epithelial misplacement--epithelial misplacement in the submucosa is marked by asterix *; ( c ) adenoma with early carcinoma; invasive carcinoma in the submucosa is marked by asterix *. HE staining, orig. magnification 4x. EPHA4: Mild positive reaction in epithelial cells in adenoma ( d ). More intensive reaction in epithelial cells and in the stroma in epithelial misplacement ( e ). Focal reaction in carcinoma cells and in the stroma of invasive carcinoma ( f ). SPON2: Positive reaction in epithelial cells and in the stroma in adenoma ( g ). Positive reaction in epithelial cells and in the stroma in epithelial misplacement ( h ). Strong reaction in carcinoma cells and positive reaction in the stroma of invasive carcinoma ( i ). SPP1: Positive reaction in epithelial cells and mild reaction in the stroma in adenoma ( j ). Positive reaction in epithelial cells and in the stroma in epithelial misplacement ( k ). Strong reaction in carcinoma cells and in the stroma of invasive carcinoma ( l ). Immunohistochemistry, orig. magnification 10x.