Explore
Validate
Learn
Western blot
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN259625 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3) (pSer552) antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified
- Reactivity
- Human, Mouse, Rat, Rabbit, Simian
- Host
- Mouse
- Epitope
- pSer552
- Isotype
- IgG
- Antibody clone number
- 14D5
- Vial size
- 0.1 mL
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
- Handling
- Avoid freeze-thaw cycles
Submitted references Phospho-NHE3 forms membrane patches and interacts with beta-actin to sense and maintain constant direction during cell migration.
NHE3 phosphorylation via PKCη marks the polarity and orientation of directionally migrating cells.
Gastrin induces sodium-hydrogen exchanger 3 phosphorylation and mTOR activation via a phosphoinositide 3-kinase-/protein kinase C-dependent but AKT-independent pathway in renal proximal tubule cells derived from a normotensive male human.
Natriuretic effect by exendin-4, but not the DPP-4 inhibitor alogliptin, is mediated via the GLP-1 receptor and preserved in obese type 2 diabetic mice.
Persistent directional cell migration requires ion transport proteins as direction sensors and membrane potential differences in order to maintain directedness.
NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphatase.
NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity.
Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites.
Perike S, Özkucur N, Sharma P, Staroske W, Bläsche R, Barth K, Funk RH
Experimental cell research 2014 May 15;324(1):13-29
Experimental cell research 2014 May 15;324(1):13-29
NHE3 phosphorylation via PKCη marks the polarity and orientation of directionally migrating cells.
Özkucur N, Song B, Bola S, Zhang L, Reid B, Fu G, Funk RHW, Zhao M
Cellular and molecular life sciences : CMLS 2014 Dec;71(23):4653-4663
Cellular and molecular life sciences : CMLS 2014 Dec;71(23):4653-4663
Gastrin induces sodium-hydrogen exchanger 3 phosphorylation and mTOR activation via a phosphoinositide 3-kinase-/protein kinase C-dependent but AKT-independent pathway in renal proximal tubule cells derived from a normotensive male human.
Liu T, Jose PA
Endocrinology 2013 Feb;154(2):865-75
Endocrinology 2013 Feb;154(2):865-75
Natriuretic effect by exendin-4, but not the DPP-4 inhibitor alogliptin, is mediated via the GLP-1 receptor and preserved in obese type 2 diabetic mice.
Rieg T, Gerasimova M, Murray F, Masuda T, Tang T, Rose M, Drucker DJ, Vallon V
American journal of physiology. Renal physiology 2012 Oct;303(7):F963-71
American journal of physiology. Renal physiology 2012 Oct;303(7):F963-71
Persistent directional cell migration requires ion transport proteins as direction sensors and membrane potential differences in order to maintain directedness.
Ozkucur N, Perike S, Sharma P, Funk RH
BMC cell biology 2011 Jan 22;12:4
BMC cell biology 2011 Jan 22;12:4
NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphatase.
Dynia DW, Steinmetz AG, Kocinsky HS
American journal of physiology. Renal physiology 2010 Mar;298(3):F745-53
American journal of physiology. Renal physiology 2010 Mar;298(3):F745-53
NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity.
Kocinsky HS, Dynia DW, Wang T, Aronson PS
American journal of physiology. Renal physiology 2007 Jul;293(1):F212-8
American journal of physiology. Renal physiology 2007 Jul;293(1):F212-8
Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites.
Kocinsky HS, Girardi AC, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, Aronson PS
American journal of physiology. Renal physiology 2005 Aug;289(2):F249-58
American journal of physiology. Renal physiology 2005 Aug;289(2):F249-58
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
