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- References [10]
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- Immunohistochemistry [10]
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- Product number
- NBP1-82574 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-82574, RRID:AB_11038394
- Product name
- Rabbit Polyclonal NHE3/SLC9A3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human NHE3/SLC9A3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Fully synthetic matrices for in vitro culture of primary human intestinal enteroids and endometrial organoids.
NHERF3 is necessary for Escherichia coli heat-stable enterotoxin-induced inhibition of NHE3: differences in signaling in mouse small intestine and Caco-2 cells.
Brush border protocadherin CDHR2 promotes the elongation and maximized packing of microvilli in vivo.
Molecular Basis and Differentiation-Associated Alterations of Anion Secretion in Human Duodenal Enteroid Monolayers.
Reversible deficits in apical transporter trafficking associated with deficiency in diacylglycerol acyltransferase.
Loss of MYO5B Leads to Reductions in Na+ Absorption With Maintenance of CFTR-Dependent Cl- Secretion in Enterocytes.
Cholera toxin inhibits SNX27-retromer-mediated delivery of cargo proteins to the plasma membrane.
Identifying the localization and exploring a functional role for Gprc5c in the kidney.
Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology.
Human Clostridium difficile infection: inhibition of NHE3 and microbiota profile.
Hernandez-Gordillo V, Kassis T, Lampejo A, Choi G, Gamboa ME, Gnecco JS, Brown A, Breault DT, Carrier R, Griffith LG
Biomaterials 2020 Sep;254:120125
Biomaterials 2020 Sep;254:120125
NHERF3 is necessary for Escherichia coli heat-stable enterotoxin-induced inhibition of NHE3: differences in signaling in mouse small intestine and Caco-2 cells.
Chen T, Lin R, Avula L, Sarker R, Yang J, Cha B, Tse CM, McNamara G, Seidler U, Waldman S, Snook A, Bijvelds MJC, de Jonge HR, Li X, Donowitz M
American journal of physiology. Cell physiology 2019 Oct 1;317(4):C737-C748
American journal of physiology. Cell physiology 2019 Oct 1;317(4):C737-C748
Brush border protocadherin CDHR2 promotes the elongation and maximized packing of microvilli in vivo.
Pinette JA, Mao S, Millis BA, Krystofiak ES, Faust JJ, Tyska MJ
Molecular biology of the cell 2019 Jan 1;30(1):108-118
Molecular biology of the cell 2019 Jan 1;30(1):108-118
Molecular Basis and Differentiation-Associated Alterations of Anion Secretion in Human Duodenal Enteroid Monolayers.
Yin J, Tse CM, Avula LR, Singh V, Foulke-Abel J, de Jonge HR, Donowitz M
Cellular and molecular gastroenterology and hepatology 2018;5(4):591-609
Cellular and molecular gastroenterology and hepatology 2018;5(4):591-609
Reversible deficits in apical transporter trafficking associated with deficiency in diacylglycerol acyltransferase.
Schlegel C, Lapierre LA, Weis VG, Williams JA, Kaji I, Pinzon-Guzman C, Prasad N, Boone B, Jones A, Correa H, Levy SE, Han X, Wang M, Thomsen K, Acra S, Goldenring JR
Traffic (Copenhagen, Denmark) 2018 Nov;19(11):879-892
Traffic (Copenhagen, Denmark) 2018 Nov;19(11):879-892
Loss of MYO5B Leads to Reductions in Na+ Absorption With Maintenance of CFTR-Dependent Cl- Secretion in Enterocytes.
Engevik AC, Kaji I, Engevik MA, Meyer AR, Weis VG, Goldstein A, Hess MW, Müller T, Koepsell H, Dudeja PK, Tyska M, Huber LA, Shub MD, Ameen N, Goldenring JR
Gastroenterology 2018 Dec;155(6):1883-1897.e10
Gastroenterology 2018 Dec;155(6):1883-1897.e10
Cholera toxin inhibits SNX27-retromer-mediated delivery of cargo proteins to the plasma membrane.
Singh V, Yang J, Yin J, Cole R, Tse M, Berman DE, Small SA, Petsko G, Donowitz M
Journal of cell science 2018 Aug 17;131(16)
Journal of cell science 2018 Aug 17;131(16)
Identifying the localization and exploring a functional role for Gprc5c in the kidney.
Rajkumar P, Cha B, Yin J, Arend LJ, Păunescu TG, Hirabayashi Y, Donowitz M, Pluznick JL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Apr;32(4):2046-2059
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Apr;32(4):2046-2059
Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology.
Foulke-Abel J, In J, Yin J, Zachos NC, Kovbasnjuk O, Estes MK, de Jonge H, Donowitz M
Gastroenterology 2016 Mar;150(3):638-649.e8
Gastroenterology 2016 Mar;150(3):638-649.e8
Human Clostridium difficile infection: inhibition of NHE3 and microbiota profile.
Engevik MA, Engevik KA, Yacyshyn MB, Wang J, Hassett DJ, Darien B, Yacyshyn BR, Worrell RT
American journal of physiology. Gastrointestinal and liver physiology 2015 Mar 15;308(6):G497-509
American journal of physiology. Gastrointestinal and liver physiology 2015 Mar 15;308(6):G497-509
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human kidney shows moderate positivity in apical membrane in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human small intestine shows strong positivity in apical membrane in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human stomach shows moderate membranous positivity in a subset of glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining in human small intestine and skeletal muscle tissues . Corresponding SLC9A3 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human cerebral cortex, colon, gallbladder and lymph node using Anti-SLC9A3 antibody NBP1-82574 (A) shows similar protein distribution across tissues to independent antibody NBP1-82575 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human cerebral cortex.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human gallbladder.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human lymph node.
