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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- NB110-74678 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-74678, RRID:AB_1049205
- Product name
- Mouse Monoclonal NHE3/SLC9A3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This antibody detects NHE3 posphorylated at serine 605, and does not cross-react with the non-phosphopeptide.
- Reactivity
- Mouse, Rat, Porcine, Rabbit, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 2.1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Resveratrol Inhibits Porcine Intestinal Glucose and Alanine Transport: Potential Roles of Na⁺/K⁺-ATPase Activity, Protein Kinase A, AMP-Activated Protein Kinase and the Association of Selected Nutrient Transport Proteins with Detergent Resistant Membranes.
NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphatase.
NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity.
Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites.
Klinger S, Breves G
Nutrients 2018 Mar 3;10(3)
Nutrients 2018 Mar 3;10(3)
NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphatase.
Dynia DW, Steinmetz AG, Kocinsky HS
American journal of physiology. Renal physiology 2010 Mar;298(3):F745-53
American journal of physiology. Renal physiology 2010 Mar;298(3):F745-53
NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity.
Kocinsky HS, Dynia DW, Wang T, Aronson PS
American journal of physiology. Renal physiology 2007 Jul;293(1):F212-8
American journal of physiology. Renal physiology 2007 Jul;293(1):F212-8
Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites.
Kocinsky HS, Girardi AC, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, Aronson PS
American journal of physiology. Renal physiology 2005 Aug;289(2):F249-58
American journal of physiology. Renal physiology 2005 Aug;289(2):F249-58
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: NHE3/SLC9A3 [p Ser605] Antibody (10A8) [NB110-74678] - Detection of phospho Ser605 in transfected COS-7 cells using NB110-74678. A. untreated B. forskolin and IBMX treated.
