Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00029883-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00029883-M01, RRID:AB_2082466
- Product name
- Mouse Monoclonal CNOT7 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CNOT7 - CCR4-NOT transcription complex, subunit 7
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Phase Transitions in the Assembly and Function of Human miRISC.
PABP Cooperates with the CCR4-NOT Complex to Promote mRNA Deadenylation and Block Precocious Decay.
CNOT3 contributes to early B cell development by controlling Igh rearrangement and p53 mRNA stability.
miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs.
MAPKAP kinase 2 blocks tristetraprolin-directed mRNA decay by inhibiting CAF1 deadenylase recruitment.
Sheu-Gruttadauria J, MacRae IJ
Cell 2018 May 3;173(4):946-957.e16
Cell 2018 May 3;173(4):946-957.e16
PABP Cooperates with the CCR4-NOT Complex to Promote mRNA Deadenylation and Block Precocious Decay.
Yi H, Park J, Ha M, Lim J, Chang H, Kim VN
Molecular cell 2018 Jun 21;70(6):1081-1088.e5
Molecular cell 2018 Jun 21;70(6):1081-1088.e5
CNOT3 contributes to early B cell development by controlling Igh rearrangement and p53 mRNA stability.
Inoue T, Morita M, Hijikata A, Fukuda-Yuzawa Y, Adachi S, Isono K, Ikawa T, Kawamoto H, Koseki H, Natsume T, Fukao T, Ohara O, Yamamoto T, Kurosaki T
The Journal of experimental medicine 2015 Aug 24;212(9):1465-79
The Journal of experimental medicine 2015 Aug 24;212(9):1465-79
miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs.
Chekulaeva M, Mathys H, Zipprich JT, Attig J, Colic M, Parker R, Filipowicz W
Nature structural & molecular biology 2011 Oct 7;18(11):1218-26
Nature structural & molecular biology 2011 Oct 7;18(11):1218-26
MAPKAP kinase 2 blocks tristetraprolin-directed mRNA decay by inhibiting CAF1 deadenylase recruitment.
Marchese FP, Aubareda A, Tudor C, Saklatvala J, Clark AR, Dean JL
The Journal of biological chemistry 2010 Sep 3;285(36):27590-600
The Journal of biological chemistry 2010 Sep 3;285(36):27590-600
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CNOT7 Antibody (2F6) [H00029883-M01] - CNOT7 monoclonal antibody (M01), clone 2F6 Analysis of CNOT7 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: CNOT7 Antibody (2F6) [H00029883-M01] - Detection limit for recombinant GST tagged CNOT7 is 3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: CNOT7 Antibody (2F6) [H00029883-M01] - Analysis of monoclonal antibody to CNOT7 on HeLa cell. Antibody concentration 40 ug/ml.