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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-31139 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CNOT7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG. Predicted reactivity: Human (99%), Mouse (99%), Rat (99%), Zebrafish (94%), Xenopus laevis (95%), Chicken (99%), Rhesus Monkey (99%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.84 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Saccharomyces cerevisiae Pop2 (CNOT7) was performed by loading 0.92 µg (Lane 1) or 1.92 µg (Lane 2) of E.coli-purified recombinant protein per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat dry milk in TBS for 2 hours at 4°C. The membrane was probed using a CNOT7 polyclonal antibody (Product # PA5-31139) at a dilution of 1:2000 in TBST overnight at 4°C, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:5000 for 2 hours at 4°C. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CNOT7 Polyclonal Antibody (Product # PA5-31139). Sample (30 µg of whole cell lysate). Lane A: U87-MG. 12% SDS PAGE. CNOT7 Polyclonal Antibody (Product # PA5-31139) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CNOT7 (Product # PA5-31139) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
