Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-58585 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSPC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PAMGPEGAAN MGTPMMPDNG AVHNDRFPQG PPSQMGSPMG SRTGSETPQA PMSGVGPVSG GPGGFGRGSQ GGNFEGPNKR RRY Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 96%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.08 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PSPC1 in mouse cell line NIH-3T3 and rat cell line NBT-II using a PSPC1 Polyclonal Antibody (Product # PA5-58585).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PSPC1 in human cell lines Caco-2 and HeLa using a PSPC1 Polyclonal Antibody (Product # PA5-58585). Corresponding PSPC1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PSPC1 in human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a PSPC1 Polyclonal Antibody (Product # PA5-58585).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PSPC1 in human testis using PSPC1 Polyclonal Antibody (Product # PA5-58585) shows nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PSPC1 in human kidney using PSPC1 Polyclonal Antibody (Product # PA5-58585) shows nuclear positivity in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PSPC1 in human cerebellum using PSPC1 Polyclonal Antibody (Product # PA5-58585) shows nuclear positivity.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PSPC1 in human skeletal muscle using PSPC1 Polyclonal Antibody (Product # PA5-58585) shows nuclear positivity in myocytes.