PA5-97193

antibody from Invitrogen Antibodies

Targeting: PSPC1 FLJ10955, PSP1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97193

Provider

Invitrogen Antibodies

Product name

PSPC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MMLRGNLKQV RIEKNPARLR ALESAVGESE PAAAAAMALA LAGEPAPPAP APPEDHPDEE MGFTIDIKSF; Positive Samples: BxPC-3, A-549, U-87MG, Mouse brain; Cellular Location: Cytoplasm, Nucleus, Nucleus matrix, Nucleus speckle, nucleolus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.78 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PSPC1 in extracts of various cell lines. Samples were incubated with PSPC1 Polyclonal antibody (Product # PA5-97193) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of PSPC1 in 200 μg extracts of A-549 cells. Samples were precipitated with 3 μg PSPC1 Polyclonal antibody (Product # PA5-97193). Western blot was performed from the immunoprecipitate using PSPC1 Polyclonal antibody (Product # PA5-97193) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PSPC1 in paraffin-embedded rat brain. Samples were incubated with PSPC1 Polyclonal antibody (Product # PA5-97193) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PSPC1 in paraffin-embedded human esophageal. Samples were incubated with PSPC1 Polyclonal antibody (Product # PA5-97193) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PSPC1 in paraffin-embedded mouse testis. Samples were incubated with PSPC1 Polyclonal antibody (Product # PA5-97193) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PSPC1 in paraffin-embedded human esophageal. Samples were incubated with PSPC1 Polyclonal antibody (Product # PA5-97193) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of PSPC1 was performed in 200 µg extracts of A-549 cells using PSPC1 Polyclonal Antibody (Product # PA5-97193). Western blot was performed from the immunoprecipitate using PSPC1 Polyclonal Antibody at a dilution of 1:1000.