PA5-51161

antibody from Invitrogen Antibodies

Targeting: VPS37A FLJ32642, HCRP1, PQBP2, SPG53

Western blot (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-51161

Provider

Invitrogen Antibodies

Product name

VPS37A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The antibody detects endogenous levels of total VPS37A protein.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Cellular abundance of sodium phosphate cotransporter SLC20A1/PiT1 and phosphate uptake are controlled post-transcriptionally by ESCRT.

Zechner C, Henne WM, Sathe AA, Xing C, Hernandez G, Sun S, Cheong MC
The Journal of biological chemistry 2022 Jun;298(6):101945

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Genetic and chemical inhibition of ESCRT/lysosomal protein degradation pathway confirm its role as direct negative regulator of SLC20A1 protein levels. A , left , immunoblot of SLC20A1 protein levels in sgVPS37A-HEK293T and sgCHMP6-HEK293T cells with sgControl ( top panel ). Immunoblots of VPS37A ( middle panel ) and CHMP6 ( bottom panel ) were performed for assessment of gene targeting efficiency. beta-actin was used as loading control. Representative data of two experiments are shown. SLC20A1/beta-actin abundance was determined by densitometry and normalized to the control group ( top ). Right , SLC20A1 mRNA expression of sgVPS37A and sgCHMP6 cells with sgControl cells was normalized to 36B4 . n = 4. Bars represent mean +- SD. B , SLC20A1 immunoblot in HEK293T cells treated with lysosome inhibitor bafilomycin A1 (BafA1) or proteasome inhibitor MG-132 versus DMSO control for 24 h ( top panel ). beta-actin was used as loading control ( middle panel ). SLC20A1/beta-actin abundance was determined by densitometry and normalized to the DMSO group ( top ). Efficiency of BafA1 and MG-132 treatment was assessed using ubiquitin immunoblot, which was exposed for optimized visualization of smaller proteins ( bottom panel ). C , HEK293T cells were transfected with CHMP6-EGFP expression plasmid for 24 h ( green in overlayed image) to induce arrest of ESCRT-mediated protein trafficking. Cells were then stained with SLC20A1 antibody and Alexa 594-coupled secondary antibody ( magenta in ove