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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- LS-C343706 - Provider product page
- Provider
- LSBio
- Product name
- TIGAR Antibody LS-C343706
- Antibody type
- Polyclonal
- Description
- Protein A purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Western Blot of endogenous and overexpressed TIGAR using PAb to TIGAR (AT122). Cell extracts from HEK 293 (lane 1), HEK 293T (lane 2) or HEK 293T transfected with a plasmid coding for human TIGAR (lane 3) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the PAb to TIGAR (AT122) at a 1:1000 dilution for 1 hour. Proteins were visualized using a peroxidase-conjugated PAb to rabbit IgG and a chemiluminescence detection system.
