935402

antibody from BioLegend

Targeting: TIGAR C12orf5

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

935402

Provider

BioLegend

Product name

Purified anti-TIGAR

Antibody type

Monoclonal

Reactivity

Human

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W18015A

Vial size

100 µg

Concentration

0.50 mg/mL

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-TIGAR antibody (clone W18015A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker. Cell lysates were loaded in ascending order of predicted TIGAR expression; predicted expression data was obtained from Human Protein Atlas.

Conjugate

Unconjugated