MAB26291

antibody from R&D Systems

Targeting: FGF23

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB26291

Provider

R&D Systems

Product name

Mouse FGF-23 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse FGF-23 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 50% cross-reactivity with recombinant human (rh) FGF-23. In Western blots, this antibody does not cross-react with rhFGF-3, -4, -5, -7, -9, -10, -11, -12, -13, -16, -17, -18, -19, acidic, basic, rmFGF-8b, -8c, -15, or -21.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

Q9EPC2


Isotype

IgG

Antibody clone number

283507

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Fibroblast growth factor 23 is upregulated in the kidney in a chronic kidney disease rat model.

Sugiura H, Matsushita A, Futaya M, Teraoka A, Akiyama KI, Usui N, Nagano N, Nitta K, Tsuchiya K
PloS one 2018;13(3):e0191706

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Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical bone.

Sakurai A, Hasegawa T, Kudo A, Shen Z, Nagai T, Abe M, Yoshida T, Hongo H, Yamamoto T, Yamamoto T, Oda K, Freitas PHL, Li M, Sano H, Amizuka N
Biomedical research (Tokyo, Japan) 2017;38(4):257-267

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Targeted Disruption of NF1 in Osteocytes Increases FGF23 and Osteoid With Osteomalacia-like Bone Phenotype.

Kamiya N, Yamaguchi R, Aruwajoye O, Kim AJ, Kuroyanagi G, Phipps M, Adapala NS, Feng JQ, Kim HK
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2017 Aug;32(8):1716-1726

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Immunolocalization of osteocyte-derived molecules during bone fracture healing of mouse ribs.

Liu Z, Yamamoto T, Hasegawa T, Hongo H, Tsuboi K, Tsuchiya E, Haraguchi M, Abe M, Freitas PH, Kudo A, Oda K, Li M, Amizuka N
Biomedical research (Tokyo, Japan) 2016;37(2):141-51

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Dietary phosphate supplement does not rescue skeletal phenotype in a mouse model for craniometaphyseal dysplasia.

Liu Y, Dutra EH, Reichenberger EJ, Chen IP
Journal of negative results in biomedicine 2016 Oct 26;15(1):18

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Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease.

Spichtig D, Zhang H, Mohebbi N, Pavik I, Petzold K, Stange G, Saleh L, Edenhofer I, Segerer S, Biber J, Jaeger P, Serra AL, Wagner CA
Kidney international 2014 Jun;85(6):1340-50

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Renal phosphate wasting due to tumor-induced osteomalacia: a frequently delayed diagnosis.

Gore MO, Welch BJ, Geng W, Kabbani W, Maalouf NM, Zerwekh JE, Moe OW, Sakhaee K
Kidney international 2009 Aug;76(3):342-7

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

FGF-23 in Mouse Brain. FGF-23 was detected in perfusion fixed frozen sections of mouse brain (cortex) using Rat Anti-Mouse FGF-23 Monoclonal Antibody (Catalog # MAB26291) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.