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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-47619 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDO Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse CDO is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CDO in immersion fixed C2C12 mouse myoblast cell line. Samples were incubated in CDO polyclonal antibody (Product # PA5-47619) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell membranes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of C2C12 mouse myoblast cell line was stained with Sheep Anti-human CDO Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47619, filled histogram) or control antibody (open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CDO in C2C12 mouse myoblast cell line. Samples were incubated in CDO polyclonal antibody (Product # PA5-47619) or control antibody followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody.
