PA1-41457

antibody from Invitrogen Antibodies

Targeting: ZMYM2 FIM, MYM, RAMP, ZNF198

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-41457

Provider

Invitrogen Antibodies

Product name

ZMYM2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is 94% homologous to mouse, rat, bovine and chicken samples and 76% homologous to xenopus. Suggested positive control: mouse skeletal muscle lysate.

Reactivity

Human, Canine

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Hepatitis B virus X protein induces EpCAM expression via active DNA demethylation directed by RelA in complex with EZH2 and TET2.

Fan H, Zhang H, Pascuzzi PE, Andrisani O
Oncogene 2016 Feb 11;35(6):715-26

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis ofZinc fingerprotein 198 inmouse skeletal musclelysate in the 1) absence and 2) presence of immunizing peptide using a ZNF198 polyclonal antibody (Product # PA1-41457) at0.1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ZMYM2 in mouse skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide. Samples were incubated in ZMYM2 polyclonal antibody (Product # PA1-41457) using a dilution of 0.1 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 8 Increased EpCAM demethylation in HBV replicating HepAD38 cells A. Diagram of CpG dinucleotides in human EpCAM gene. The transcriptional start site (TSS), numbered CpG dinucleotides, a NF-kappaB site, and the position of bisulfite sequencing primers (BSP) are indicated. B. Immunoblots of nuclear extracts isolated from HepAD38 cells grown with (+) or without (-) HBV replication by tetracycline removal for 5 and 10 days, employing antibodies for ZNF198, SUZ12, EED, EZH2, HBc, and Histone3 serving as loading control. * indicates non-specific band. C. Immunoblots of HepAD38 lysates from cells grown with (+) or without (-) Tet for 5 and 10 days, employing the indicated antibodies. D . ChIP assays with H3K27me3 antibody employing primers for the human EpCAM CpG island. E. Bisulfite sequencing results of EpCAM clones, using DNA from HepAD38 cells gown in the absence (-) of HBV replication or with (+) HBV replication for 2 days, by tetracycline removal. % methylation is indicated. Arrows indicate CpG dinucleotide #23 adjacent to NF-kappaB site.