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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [3]
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- Product number
- R34689 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NCAM2 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific NCAM2 antibody is suitable for use in Immunohistochemistry/ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the NCAM2 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC: 48h-staining of PFA-perfused cryosection of mouse olfactory bulb with NCAM2 antibody at 0.5ug/ml. HIER: pH6 citrate buffer at 95oC for 10min. Staining: NCAM2 (red), VGLUT2 (blue) and NQO1 (green).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC 24h-staining of a PFA-perfused cryosection of mouse olfactory bulb with NCAM2 antibody at 0.5ug/ml. Strong staining of axon bundles (right middle) and moderate peripheral staining outside the glomeruli (top) is seen. Ag retrieval: methanol (-20oC, 10min), then 1% SDS (10min).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human cortex (brain) tissue with NCAM2 antibody at 3.75ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
