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Western blot
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- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- HPA036227 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA036227, RRID:AB_10696653
- Product name
- Anti-SLC7A7
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human SLC7A7, Gene description: solute carrier family 7 (amino acid transporter light chain, y+L system), member 7, Alternative Gene Names: LPI, y+LAT-1, Validated applications: IHC, WB, Uniprot ID: Q9UM01, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references
Expression profile of the amino acid transporters
SLC
7A5,
SLC
7A7,
SLC
7A8 and the enzyme
TDO
2 in basal cell carcinoma
Tina E, Prosén S, Lennholm S, Gasparyan G, Lindberg M, Göthlin Eremo A
British Journal of Dermatology 2018;180(1):130-140
British Journal of Dermatology 2018;180(1):130-140
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and SLC7A7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418308).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human kidney and liver tissues using HPA036227 antibody. Corresponding SLC7A7 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
