Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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Validation data
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- Product number
- 16217-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDGFRL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MKVWLLLGL LLVHEALEDV TGQHLPKNKR PKEPGENRIK PTNKKVKPKI PKMKDRDSAN SAPKTQSIMM QVLDKGRFQK PAATLSLLAG QTVELRCKGS RIGWSYPAYL DTFKDSRLSV KQNERYGQLT LVNSTSADTG EFSCWVQLCS GYICRKDEAK TGSTYIFFTE KGELFVPSPS YFDVVYLNPD RQAVVPCRVT VLSAKVTLHR EFPAKEIPAN GTDIVYDMKR GFVYLQPHSE HQGVVYCRAE AGGRSQISVK YQLLYVAVPS GPPSTTILAS SNKVKSGDDI SVLCTVLGEP DVEVEFTWIF PGQKDERPVT IQDTWRLIHR GLGHTTRISQ SVITVEDFET IDAGYYICTA QNLQGQTTVA TTVEFS (1-375 aa encoded by B C010927)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.45 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 16217-1-AP ( PDGFRL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 16217-1-AP (PDGFRL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of L02 cells using 16217-1-AP (PDGFRL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse liver tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse liver tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16217-1-AP ( PDGFRL Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16217-1-AP ( PDGFRL Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with .2ug PDGFRL antibody (16217-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.