H00029121-M01

antibody from Novus Biologicals

Targeting: CLEC2D CLAX, LLT1, OCIL

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

H00029121-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00029121-M01, RRID:AB_2229777

Product name

Mouse Monoclonal OCIL/CLEC2d Antibody

Antibody type

Monoclonal

Description

IgG purified. CLEC2D - C-type lectin superfamily 2, member D

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Vaccinia virus Western Reserve induces rapid surface expression of a host molecule detected by the antibody 4C7 that is distinct from CLEC2D.

Williams KJ, Eaton HE, Jones L, Rengan S, Burshtyn DN
Microbiology and immunology 2016 Nov;60(11):754-769

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Expression of Lectin-Like Transcript 1, the Ligand for CD161, in Rheumatoid Arthritis.

Chalan P, Bijzet J, Huitema MG, Kroesen BJ, Brouwer E, Boots AM
PloS one 2015;10(7):e0132436

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Respiratory syncytial virus infection, TLR3 ligands, and proinflammatory cytokines induce CD161 ligand LLT1 expression on the respiratory epithelium.

Satkunanathan S, Kumar N, Bajorek M, Purbhoo MA, Culley FJ
Journal of virology 2014 Mar;88(5):2366-73

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Induction of lectin-like transcript 1 (LLT1) protein cell surface expression by pathogens and interferon-γ contributes to modulate immune responses.

Germain C, Meier A, Jensen T, Knapnougel P, Poupon G, Lazzari A, Neisig A, Håkansson K, Dong T, Wagtmann N, Galsgaard ED, Spee P, Braud VM
The Journal of biological chemistry 2011 Nov 4;286(44):37964-75

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Characterization of alternatively spliced transcript variants of CLEC2D gene.

Germain C, Bihl F, Zahn S, Poupon G, Dumaurier MJ, Rampanarivo HH, Padkjær SB, Spee P, Braud VM
The Journal of biological chemistry 2010 Nov 12;285(46):36207-15

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Functional consequences of interactions between human NKR-P1A and its ligand LLT1 expressed on activated dendritic cells and B cells.

Rosen DB, Cao W, Avery DT, Tangye SG, Liu YJ, Houchins JP, Lanier LL
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6508-17

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Functional consequences of interactions between human NKR-P1A and its ligand LLT1 expressed on activated dendritic cells and B cells.

Rosen DB, Cao W, Avery DT, Tangye SG, Liu YJ, Houchins JP, Lanier LL
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6508-17

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Malignant glioma cells counteract antitumor immune responses through expression of lectin-like transcript-1.

Roth P, Mittelbronn M, Wick W, Meyermann R, Tatagiba M, Weller M
Cancer research 2007 Apr 15;67(8):3540-4

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: OCIL/CLEC2d Antibody (4C7) [H00029121-M01] - Analysis of CLEC2D expression in transfected 293T cell line by CLEC2D monoclonal antibody (M01), clone 4C7.Lane 1: CLEC2D transfected lysate(21.8 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: OCIL/CLEC2d Antibody (4C7) [H00029121-M01] - Detection limit for recombinant GST tagged CLEC2D is 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: OCIL/CLEC2d Antibody (4C7) [H00029121-M01] - Analysis of monoclonal antibody to CLEC2D on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.