PA5-142472

antibody from Invitrogen Antibodies

Targeting: CLEC2D CLAX, LLT1, OCIL

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-142472

Provider

Invitrogen Antibodies

Product name

CLEC2D Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Additional Information: This antibody is expected to recognize all isoforms (NP_037401.1; NP_001004419.1; NP_001184246.1). This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:16,000.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of CLEC2D in A431 cells using CLEC2D Polyclonal Antibody (Product # PA5-142472). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of CLEC2D in A431 cells using CLEC2D Polyclonal Antibody (Product # PA5-142472). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CLEC2D in human tonsil. Samples were incubated with CLEC2D polyclonal antibody (Product # PA5-142472) using a dilution of 3.75 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CLEC2D in human placenta. Samples were incubated with CLEC2D polyclonal antibody (Product # PA5-142472) using a dilution of 3.75 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of CLEC2D in Jurkat cells (blue line) using CLEC2D Polyclonal Antibody (Product # PA5-142472). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of CLEC2D in Jurkat cells (blue line) using CLEC2D Polyclonal Antibody (Product # PA5-142472). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.