PA5-28779

antibody from Invitrogen Antibodies

Targeting: MPST MST, TST2, TUM1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28779

Provider

Invitrogen Antibodies

Product name

MPST Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, mouse liver. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.57 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

N-Acetylcysteine Serves as Substrate of 3-Mercaptopyruvate Sulfurtransferase and Stimulates Sulfide Metabolism in Colon Cancer Cells.

Zuhra K, Tomé CS, Masi L, Giardina G, Paulini G, Malagrinò F, Forte E, Vicente JB, Giuffrè A
Cells 2019 Aug 4;8(8)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MPST was performed by separating 50 µg of mouse tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a MPST Polyclonal Antibody (Product # PA5-28779) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using MPST Polyclonal Antibody (Product # PA5-28779). Sample (30 µg of whole cell lysate). A: A431. 12% SDS PAGE. MPST Polyclonal Antibody (Product # PA5-28779) diluted at 1:500.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using MPST (Product # PA5-28779) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Effect of NAC on MST and SQR expression. (Panel A ) Representative Western blot analysis of MST expression in SW480 cells grown for 24 h in DMEM alone (control) or supplemented with 10 mM NAC (NAC). The blots are shown together with their corresponding total protein load quantitation by stain-free imaging technology (see Materials and Methods). (Panel B ) MST expression levels as normalized to total protein. Data represent the mean value +- SEM of 6 repeats, each carried out in technical duplicate. *** p