Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-85133 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85133, RRID:AB_11002763
- Product name
- Rabbit Polyclonal KCC4/SLC12A7 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human KCC4/SLC12A7 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Modulation of brain cation-Cl(-) cotransport via the SPAK kinase inhibitor ZT-1a.
SLC12A7 alters adrenocortical carcinoma cell adhesion properties to promote an aggressive invasive behavior.
Zhang J, Bhuiyan MIH, Zhang T, Karimy JK, Wu Z, Fiesler VM, Zhang J, Huang H, Hasan MN, Skrzypiec AE, Mucha M, Duran D, Huang W, Pawlak R, Foley LM, Hitchens TK, Minnigh MB, Poloyac SM, Alper SL, Molyneaux BJ, Trevelyan AJ, Kahle KT, Sun D, Deng X
Nature communications 2020 Jan 7;11(1):78
Nature communications 2020 Jan 7;11(1):78
SLC12A7 alters adrenocortical carcinoma cell adhesion properties to promote an aggressive invasive behavior.
Brown TC, Murtha TD, Rubinstein JC, Korah R, Carling T
Cell communication and signaling : CCS 2018 Jun 8;16(1):27
Cell communication and signaling : CCS 2018 Jun 8;16(1):27
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KCC4/SLC12A7 Antibody [NBP1-85133] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KCC4/SLC12A7 Antibody [NBP1-85133] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KCC4/SLC12A7 Antibody [NBP1-85133] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KCC4/SLC12A7 Antibody [NBP1-85133] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KCC4/SLC12A7 Antibody [NBP1-85133] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KCC4/SLC12A7 Antibody [NBP1-85133] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.