PA5-67275

antibody from Invitrogen Antibodies

Targeting: NDUFA9 CI-39k, COQ11, NDUFS2L, SDR22E1

Antibody data

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Validation data

Product number: PA5-67275 - Provider product page
Provider: Invitrogen Antibodies
Product name: NDUFA9 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: AQLSKEAGVEK FIHVSHLNAN IKSSSRYLRN KAVGEKVVRD AFPEAIIVKP SDIFGREDRF LNSFASMHRF GPIPL
Concentration: 0.05 mg/mL

NDUFA9 protein structure - PA5-67275 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NDUFA9 in human cell line RT-4 and human cell line U-251 MG. Samples were probed using a NDUFA9 Polyclonal Antibody (Product # PA5-67275).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of NDUFA9 was achieved by transfecting Hep G2 cells with NDUFA9 specific siRNAs (Silencer® select Product # s39346, s9347). Western blot analysis (Fig. a) was performed using membrane enriched cell extracts from the NDUFA9 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed NDUFA9 Polyclonal Antibody (Product # PA5-67275, 1:500 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 ug/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to NDUFA9.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using NDUFA9 Polyclonal Antibody (Product # PA5-67275) and a 36 kDa band corresponding to NDUFA9 was observed across cell lines and tissues tested. Membrane enriched cell extracts (30 µg lysate) of HeLa (Lane 1), Caco-2 (Lane 2), SW480 (Lane 3), Hep G2 (Lane 4), Jurkat (Lane 5), K-562 (Lane 6), MCF-7 (Lane 7), Mouse Liver (Lane 8), Rat Liver (Lane 9), Mouse Brain (Lane 10) and Rat Brain (Lane 11) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).