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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00002267-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00002267-M01, RRID:AB_425432
- Product name
- FGL1 monoclonal antibody (M01), clone 2A4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant FGL1.
- Antigen sequence
EISALEDCAQEQMRLRAQVRLLETRVKQQQVKIKQ
LLQENEVQFLDKGDENTVIDLGSKRQYADCSEIFN
DGYKLSGFYKIKPLQSPAEFSVYCDMSDGGGWTVI
QRRSDGSENFNRGWKDYENGFGNFVQKHGEYWLGN
KNLHFLTTQEDYTLKIDLADFEKNSRYAQYKNFKV
GDEKNFYELNIGEYSGTAGDSLAGNFHPEVQWWAS
HQRMKFSTWDRDHDNYEGNCAEEDQSGWWFNRCHS
ANLNGVYYSGPYTAKTDNGIVWYTWHGWWYSLKSV
VMKIRPNDFIPNVI- Isotype
- IgG
- Antibody clone number
- 2A4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of FGL1 expression in transfected 293T cell line by FGL1 monoclonal antibody (M01), clone 2A4.Lane 1: FGL1 transfected lysate(36.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged FGL1 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of FGL1 transfected lysate using anti-FGL1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with FGL1 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
