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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- 67391-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FGL1 Monoclonal Antibody (2B11C6)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Aliquoting is unnecessary for -20°C storage.
- Reactivity
- Human, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2B11C6
- Vial size
- 150 µL
- Concentration
- 913 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Pig liver tissue were subjected to SDS PAGE followed by western blot with 67391-1-Ig (FGL1 antibody) at dilution of 1:2700 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67391-1-Ig (FGL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67391-1-Ig (FGL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were intracellularly stained with 0.2 µg Anti-Human FGL1 (67391-1-Ig, Clone:2B11C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 µg Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.
