Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA324490 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal ATP5J Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal ATP5J Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ATP5PF
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ATP5J Antibody (Center) (Cat. #TA324490) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the ATP5J antibody detected the ATP5J protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of U251 cell stained with ATP5J Antibody (Center)(Cat#TA324490).U251 cells were incubated with ATP5J primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .ATP5J immunoreactivity is localized to Mitochondria significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ATP5J Antibody (Center) (Cat. #TA324490)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ATP5J Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
