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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-50201 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP5J Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The antibody detects endogenous levels of total ATP5J protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.6 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP5J was performed by loading (from left to right): Human fetal brain tissue and 293T cell, mouse intestinum tenue tissue and lovo cell lysates (40µg) on to a 12% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a ATP5J antibody (Product # PA5-50201) at a 1/300 dilution for 1 minute, followed by a secondary antibody at 1/8000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ATP5J in paraffin embedded Human colon cancer tissue using ATP5J Polyclonal Antibody (Product # PA5-50201) at a 1:15 dilution.
