PA5-29202

antibody from Invitrogen Antibodies

Targeting: ATP5PF ATP5, ATP5A, ATP5J, ATPM, CF6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29202

Provider

Invitrogen Antibodies

Product name

ATP5J Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: HeLa, HeLa membrane. Predicted reactivity: Mouse (80%), Rhesus Monkey (91%), Bovine (80%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ATP5J Polyclonal Antibody (Product # PA5-29202). Sample (30 µg of whole cell lysate). Lane A: HeLa. Lane B: HeLa membrane . 15% SDS PAGE. ATP5J Polyclonal Antibody (Product # PA5-29202) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ATP5J Polyclonal Antibody (Product # PA5-29202). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATP5J Polyclonal Antibody (Product # PA5-29202) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2) and Mouse Stomach (Lane 3). The blot was probed with Anti-ATP5J Polyclonal Antibody (Product # PA5-29202, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 10 kDa band corresponding to ATP5J was detected in Mouse Heart, Rat Heart and not in Mouse Stomach which is reported negative for ATP5J expression. An uncharacterized band was observed at 60 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ATP5J in methanol-fixed HeLa cells using an ATP5J polyclonal antibody (Product # PA5-29202) at a 1:500 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ATP5J was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ATP5J Polyclonal Antibody (Product # PA5-29202) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of ATP5J was performed in paraffin-embedded mouse duodenum tissue using ATP5J Polyclonal Antibody (Product # PA5-29202) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of ATP5J was performed in paraffin-embedded mouse kidney tissue using ATP5J Polyclonal Antibody (Product # PA5-29202) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using ATP5J (Product # PA5-29202) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.