PA5-109341

antibody from Invitrogen Antibodies

Targeting: KCNA1 AEMK, HUK1, Kv1.1, MBK1, RBK1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109341

Provider

Invitrogen Antibodies

Product name

KV1.1 (KCNA1) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Control: Rat brain tissue lysate, rat spinal cord tissue, mouse brain tissue, A549.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KV1.1 in rat brain tissue lysate. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with KV1.1 polyclonal antibody (Product # PA5-109341), at a dilution of 1:500, followed by Goat Anti-Rabbit IgG-HRP (1 hour) with a dilution of 1:5000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KV1.1 in paraffin-embedded rat spinal cord tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with KV1.1 polyclonal antibody (Product # PA5-109341) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KV1.1 in paraffin-embedded mouse brain tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with KV1.1 polyclonal antibody (Product # PA5-109341) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of KV1.1 in A549 cells, unlabelled sample (control; cells without incubation with primary antibody; black). Samples were incubated with KV1.1 polyclonal antibody (Product # PA5-109341) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG with a dilution of 1:1000 (30 min).