Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- R34499 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- IFT88 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific IFT88 antibody is suitable for use in Immunohistochemistry/Immunofluorescence/Flow cytometry/ELISA applications with human and rat samples.
- Reactivity
- Human, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the IFT88 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of A) human K562 cell lysate without and with blocking peptide, B) human ThP-1 cell lysate without and with blocking peptide, C) human U-251 cell lysate without and with blocking peptide and D) rat KNRK cell lysate without and with blocking peptide, with IFT88 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human HepG2 cells with ITF88 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human A549 cells with ITF88 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human kidney with IFT88 antibody at 3ug/ml. HIER: microwaved with pH9 Tris/EDTA buffer, HRP-staining.