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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Blocking/Neutralizing [1]
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- Product number
- MAB267-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human NT-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human NT-3 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) BDNF, rhCNTF, rhGDNF, rh beta-NGF, and rhNT-4 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P20783- Isotype
- IgG
- Antibody clone number
- 41512
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Neurotrophin-3 production promotes human neuroblastoma cell survival by inhibiting TrkC-induced apoptosis.
Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.
The neurotrophins nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4 are survival and activation factors for eosinophils in patients with allergic bronchial asthma.
Bouzas-Rodriguez J, Cabrera JR, Delloye-Bourgeois C, Ichim G, Delcros JG, Raquin MA, Rousseau R, Combaret V, Bénard J, Tauszig-Delamasure S, Mehlen P
The Journal of clinical investigation 2010 Mar;120(3):850-8
The Journal of clinical investigation 2010 Mar;120(3):850-8
Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.
Samah B, Porcheray F, Gras G
Clinical and experimental immunology 2008 Mar;151(3):476-86
Clinical and experimental immunology 2008 Mar;151(3):476-86
The neurotrophins nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4 are survival and activation factors for eosinophils in patients with allergic bronchial asthma.
Nassenstein C, Braun A, Erpenbeck VJ, Lommatzsch M, Schmidt S, Krug N, Luttmann W, Renz H, Virchow JC Jr
The Journal of experimental medicine 2003 Aug 4;198(3):455-67
The Journal of experimental medicine 2003 Aug 4;198(3):455-67
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by NT-3 and Neutralization by Human NT-3 Antibody. Recombinant Human NT-3 (Catalog # 267-N3) stimulates proliferation in BaF-TrKB-BD mouse pro-B cell line transfected with TrkB in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NT-3 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human NT-3 Monoclonal Antibody (Catalog # MAB267). The ND50 is typically 0.1-0.5 µg/mL.
