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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-47613 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47613, RRID:AB_10010570
- Product name
- Mouse Monoclonal GABA-A R beta 3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 55kDa. No cross-reactivity against GABA-A-R-Beta 2 or -Beta1.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references GABAA Receptors Are Well Preserved in the Hippocampus of Aged Mice.
Sex- and age-related changes in GABA signaling components in the human cortex.
GABAergic signaling in primary lens epithelial and lentoid cells and its involvement in intracellular Ca2+ modulation.
Palpagama TH, Sagniez M, Kim S, Waldvogel HJ, Faull RL, Kwakowsky A
eNeuro 2019 Jul Aug;6(4)
eNeuro 2019 Jul Aug;6(4)
Sex- and age-related changes in GABA signaling components in the human cortex.
Pandya M, Palpagama TH, Turner C, Waldvogel HJ, Faull RL, Kwakowsky A
Biology of sex differences 2019 Jan 14;10(1):5
Biology of sex differences 2019 Jan 14;10(1):5
GABAergic signaling in primary lens epithelial and lentoid cells and its involvement in intracellular Ca2+ modulation.
Schwirtlich M, Kwakowsky A, Emri Z, Antal K, Lacza Z, Cselenyák A, Katarova Z, Szabó G
Cell calcium 2011 Oct;50(4):381-92
Cell calcium 2011 Oct;50(4):381-92
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GABA-A R beta 3 Antibody (S87-25) [NBP1-47613] - Analysis of Rat brain membrane lysate showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S87-25 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GABA-A R beta 3 Antibody (S87-25) [NBP1-47613] - Tissue: Brain. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.
