A02804

antibody from Boster Biological Technology

Targeting: HCN2 BCNG-2, BCNG2, HAC-1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A02804

Provider

Boster Biological Technology

Product name

Anti-HCN2 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for HCN2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. HCN2 information: Molecular Weight: 96950 MW; Subcellular Localization: Cell membrane ; Multi-pass membrane protein ; Tissue Specificity: Highly expressed throughout the brain. Detected at low levels in heart.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

The HCN domain is required for HCN channel cell-surface expression and couples voltage- and cAMP-dependent gating mechanisms.

Wang ZJ, Blanco I, Hayoz S, Brelidze TI
The Journal of biological chemistry 2020 Jun 12;295(24):8164-8173

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Spironolactone diminishes spontaneous ventricular premature beats by reducing HCN4 protein expression in rats with myocardial infarction.

Song T, Yang J, Yao Y, Li H, Chen Y, Zhang J, Huang C
Molecular medicine reports 2011 May-Jun;4(3):569-73

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Dynamic changes in HCN2, HCN4, KCNE1, and KCNE2 expression in ventricular cells from acute myocardial infarction rat hearts.

Xia S, Wang Y, Zhang Y, Deng SB, Du JL, Wang XC, She Q
Biochemical and biophysical research communications 2010 May 7;395(3):330-5

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of HCN2 using anti- HCN2 antibody (A02804). HCN2 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- HCN2 Antibody (A02804) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image