NBP1-05970

HERC2 antibody from Novus Biologicals

D15F37S1, jdf2, p528

Western blot

Supportive data in Antibodypedia

Immunoprecipitation

Supportive data in Antibodypedia

Provider product page for NBP1-05970

Antibody Data

Product number

NBP1-05970

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-05970, RRID:AB_1555329

Product name

Rabbit Polyclonal HERC2 Antibody

Provider product page

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HERC2 Antibody [NBP1-05970] - Detection of Mouse HERC2- Whole cell lysate (50 ug) from NIH3T3, TCMK-1, Renca, 4T1, and CT26.WT cells. Affinity purified rabbit anti-HERC2 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: HERC2 Antibody [NBP1-05970] - Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-HERC2 antibody used for WB. HERC2 was also immunoprecipitated by another rabbit anti-HERC2 antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: HERC2 Antibody [NBP1-05970] - Detection of human HERC2 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HERC2 antibody NBP1-05970 (lot NBP1-05970-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HERC2 was also immunoprecipitated by a previous lot (lot NBP1-05970-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).