MAB2530

antibody from Novus Biologicals

Targeting: PVR CD155, HVED, Necl-5, NECL5, PVS, Tage4

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2530

Provider

Novus Biologicals

Product name

Mouse Monoclonal CD155/PVR Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human CD155/PVR in direct ELISAs and Western blots.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

The Cell Adhesion Molecule Necl-4/CADM4 Serves as a Novel Regulator for Contact Inhibition of Cell Movement and Proliferation.

Yamana S, Tokiyama A, Mizutani K, Hirata K, Takai Y, Rikitake Y
PloS one 2015;10(4):e0124259

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human CD155/PVR by Western Blot. Western blot shows lysates of human heart tissue, HT1080 human fibrosarcoma cell line, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD155/PVR Monoclonal Antibody (Catalog # MAB2530) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD155/PVR at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.