46-1550-42

antibody from Invitrogen Antibodies
Targeting: PVR CD155, HVED, Necl-5, NECL5, PVS, Tage4
Provider product page for 46-1550-42

Antibody data

Product number
46-1550-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD155 Monoclonal Antibody (2H7CD155), PerCP-eFluor™ 710, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal antibody 2H7CD155 recognizes CD155 also known as the poliovirus receptor. The CD155 protein is a member of the Ig superfamily in which 4 isoforms exist; only the alpha and delta forms are expressed on the membrane of monocytes, prostate cancer cells, as well as neurectodermal and monocytic tumor cells lines. In addition, CD155 is found during mid-gastrulation during embryonic development. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD226 and CD96 are ligands for CD155. Blocking of CD155 is under therapeutic investigation for prevention of variants of poliovirus. Applications Reported: This 2H7CD155 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2H7CD155 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells or human tumor cell line U937. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
2H7CD155
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
PVR protein structure - 46-1550-42 shown in red.