AF2530

antibody from R&D Systems

Targeting: PVR CD155, HVED, Necl-5, NECL5, PVS, Tage4

Antibody data

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Validation data

Product number: AF2530 - Provider product page
Provider: R&D Systems
Product name: Human CD155/PVR Antibody
Antibody type: Polyclonal
Description: Antigen Affinity-purified. Detects human CD155/PVR in direct ELISAs and Western blots.
Reactivity: Human
Host: Goat
Conjugate: Unconjugated
Antigen sequence: AAH15542
Isotype: IgG
Vial size: 100 ug
Concentration: LYOPH
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

PVR protein structure - AF2530 shown in red.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human CD155/PVR by Western Blot. Western blot shows lysates of human heart tissue, HT1080 human fibrosarcoma cell line, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human CD155/PVR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2530) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD155/PVR at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Detection of Human CD155/PVR by Simple Western^TM. Simple Western lane view shows lysates of HT1080 human fibrosarcoma cell line and HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for CD155/PVR at approximately 128 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human CD155/PVR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2530) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by: R&D Systems (provider)
Main image
Experimental details: Western Blot Shows Human CD155/PVR Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and CD155/PVR knockout HEK293T cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human CD155/PVR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2530) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD155/PVR at approximately 75 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.