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- Antibody Data
- Antigen structure
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- Other assay [1]
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- Product number
- PA5-47115 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FLRG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse FLRG is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Neutralization of FLRG in K562 human chronic myelogenous leukemia cell line. Samples were incubated in FLRG polyclonal antibody (Product # PA5-47115). Recombinant Human Follistatin-related Gene Protein/FLRG inhibits Recombinant Human/Mouse/Rat Activin A induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase activity. Inhibition of Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) activity elicited by Recombinant Human Follistatin‚related Gene Protein/FLRG (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Follistatin-related Gene Protein/FLRG Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 1‚4 µg/mL.
