PA5-47115

antibody from Invitrogen Antibodies

Targeting: FSTL3 FLRG, FSRP

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47115

Provider

Invitrogen Antibodies

Product name

FLRG Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse FLRG is observed.

Concentration

0.2 mg/mL

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of FLRG in K562 human chronic myelogenous leukemia cell line. Samples were incubated in FLRG polyclonal antibody (Product # PA5-47115). Recombinant Human Follistatin-related Gene Protein/FLRG inhibits Recombinant Human/Mouse/Rat Activin A induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase activity. Inhibition of Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) activity elicited by Recombinant Human Follistatin‚related Gene Protein/FLRG (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Follistatin-related Gene Protein/FLRG Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 1‚4 µg/mL.