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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-31849 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRUNOL4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, HCT116. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (97%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.69 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BRUNOL4 Polyclonal Antibody (Product # PA5-31849). Sample (30 µg of whole cell lysate). Lane A: H1299. Lane B: HCT116 . 7.5% SDS PAGE. BRUNOL4 Polyclonal Antibody (Product # PA5-31849) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using BRUNOL4 (Product # PA5-31849) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
