Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-37845 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carbonic Anhydrase XII Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Carbonic Anhydrase XII/CA12 in human kidney lysate was performed using 35 µg protein in RIPA buffer. Lysates were probed with a Carbonic Anhydrase XII/CA12 polyclonal antibody (Product # PA5-37845) at a dilution of 0.3 µg/mL and incubated for 1 hour, followed by chemiluminescent detection.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Carbonic Anhydrase XII/CA12 in paraffin-embedded human kidney following steamed antigen retrieval with a citrate buffer, pH 6, using a Carbonic Anhydrase XII/CA12 polyclonal antibody (Product # PA5-37845) at a dilution of 5 µg/mL. Detection was performed using an AP-conjugated secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Carbonic Anhydrase XII in paraffin embedded human kidney using a Carbonic Anhydrase XII polyclonal antibody (Product #PA5-37845) at a concentration of 5 µg/mL. Steamed antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained with alkaline phosphatase.
