TA505570

antibody from OriGene

Targeting: CA12 HsT18816

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505570

Provider

OriGene

Proper citation

OriGene Cat#TA505570, RRID:AB_2623194

Product name

CA12 mouse monoclonal antibody, clone OTI2E9 (formerly 2E9)

Antibody type

Monoclonal

Description

CA12 mouse monoclonal antibody, clone OTI2E9 (formerly 2E9)

Host

Mouse

Conjugate

Unconjugated

Epitope

CA12

Isotype

IgG

Antibody clone number

OTI2E9

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CA12 (RC204810, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CA12.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CA12 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-CA12 mouse monoclonal antibody (TA505570) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CA12(RC204810).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-CA12 mouse monoclonal antibody (TA505570).

Validation comment

IF